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This proteolytic enzyme is derived from unripe papaya and serves for mild and effective dissociation of cell-cell or cell-matrix connections of adherent cells. Our ready-to-use papain solution (61.25 mg/l) is free of calcium and magnesium ions (the absence of divalent cations enhances enzymatic efficiency in cell detachment or tissue digestion) and extra stable (24 months stable at -20°C).
| Product | Article Number | Volume |
|---|---|---|
| Papain 61.25 mg/l dissolved in D(PBS) | SB-PAP-100 | 100 ml |
| Papain 61.25 mg/l dissolved in D(PBS) | SB-PAP-500 | 500 ml |
Gentle isolation of primary cells from tissue with preserved receptor function
Passaging sensitive stem cell lines with minimal cell stress induction
Detachment of sensitive neuronal cells without damage to neurites
Preparation of single-cell suspensions with intact surface markers
Analysis of surface receptors and signal transduction pathways
Downstream protein analyses with preserved protein integrity
Experience the quality of our Papain Solution yourself
Our Papain Solution 61.25 mg/l is the ideal choice for researchers seeking a gentle and sustainable method for cell detachment. Unlike conventional trypsin derived from animal pancreas, papain is extracted from unripe papaya and thus offers a plant-based, ethical alternative.
The proteolytic activity of papain enables mild yet effective dissociation of cell-cell and cell-matrix connections. Particularly when working with sensitive cell lines, stem cells, or primary cells, papain demonstrates its advantages: higher cell viability, better preservation of surface receptors, and more reproducible results in downstream applications.
Our ready-to-use formulation saves valuable laboratory time. The solution is free of Ca²⁺ and Mg²⁺, which significantly enhances enzymatic efficiency. With a shelf life of 24 months at -20°C, we offer you a particularly stable product for your cell culture work.
Using our Papain Solution is straightforward: First, completely remove the culture medium from your adherent cells. Wash the cells with PBS to remove serum residues. Add sufficient papain solution to completely cover the cell layer (approx. 1-2 ml per 25 cm² culture area). Incubate at 37°C for 5-15 minutes, depending on the cell line. Regularly check under the microscope whether the cells are detaching. Stop the reaction by adding serum-containing medium and use the cells for your further experiments.
Order your Papain Solution from SeamlessBio today and benefit from higher cell viability, better cell integrity, and more sustainable research. For questions about applications or larger order quantities, our expert team is always available.
Papain is a proteolytic enzyme derived from the papaya fruit, greatly valued for its ability to break down proteins. This specific Papain solution lacks calcium (Ca²⁺) and magnesium (Mg²⁺), making it particularly suitable for sensitive biological applications. It provides a clean environment for enzymatic reactions without the interference of added salts or color indicators, such as phenol red. Therefore, researchers can achieve more accurate results when utilizing this solution.
This enzyme is widely used in various fields, including biotechnology and medicine. In laboratories, it is applied in protein digestion studies and as a meat tenderizer in food science. Unlike solutions that contain Ca²⁺ and Mg²⁺, which may catalyze unwanted reactions, this Papain solution is specifically designed for optimal performance in controlled experimental settings. Hence, it offers consistent results for enzymatic reactions.
To effectively use this Papain solution, it is essential to consider the appropriate concentrations and conditions. Carefully measure the volume suitable for your application, ensuring it aligns with your experimental design. Before application, thoroughly mix the solution for uniformity. Moreover, store it according to recommended guidelines to maintain its activity. Because of its unique properties, this solution is ideal for researchers looking to explore enzymatic activity without extraneous variables.
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