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Density gradient medium optimized at 1.077 g/mL for reliable isolation of peripheral blood mononuclear cells
Lymphobio is a premium density gradient medium specifically designed for the isolation of peripheral blood mononuclear cells (PBMCs) from whole blood samples. Based on highly purified, neutral polysucrose with an optimized density of 1.077 g/mL, Lymphobio enables reproducible and high-yield separation of lymphocytes, monocytes, and other mononuclear cells.
The polysucrose-based formulation (average molecular weight 400,000 Da) is highly cross-linked, ensuring minimal osmotic effects and maximum cell viability post-isolation. This makes Lymphobio the ideal choice for downstream applications requiring intact, functional immune cells.
PBMC isolation using Lymphobio is based on the principle of density gradient centrifugation, a gold-standard method in immunology and cell biology:
The density of 1.077 g/mL is specifically optimized to separate mononuclear cells (density 1.065–1.077 g/mL) from:
This precise density ensures maximum purity and viability of isolated PBMCs.
Polysucrose formulation minimizes osmotic stress, typically yielding >95% viable PBMCs
Batch-to-batch consistency ensures reliable protocols across experiments
Sterile, no dilution or preparation required – saves time in the lab
Adjustable density up to 1.2 g/mL for specialized separations
Certificate of Analysis (CoA) available for every batch
| Parameter | Specification |
|---|---|
| Density | 1.077 ± 0.001 g/mL (at 20°C) |
| Base Material | Polysucrose (highly purified, cross-linked) |
| Molecular Weight | ~400,000 Da (average) |
| Osmolality | 290 ± 15 mOsm/kg |
| pH | 7.0 ± 0.3 |
| Sterility | Sterile filtered (0.2 µm) |
| Endotoxin | <0.25 EU/mL |
| Storage | 2–8°C, protected from light |
| Shelf Life | 24 months from production date |
| Available Sizes | 100 mL | 500 mL |
| Bottle Size | Article Number | Price (net) |
|---|---|---|
| 100 mL | SBS-LYM-100 | On request |
| 500 mL | SBS-LYM-500 | On request |
Note: Each bottle is individually batch-documented with Certificate of Analysis (CoA).
2–8°C (refrigerated)
24 months from production date
Store in original bottle, protected from light
Freezing may alter density properties
Typical yield is 1–2 × 10⁶ PBMCs per mL of whole blood, depending on donor health and sample quality. Cell viability is typically >95%.
Yes, Lymphobio works with EDTA, heparin, or citrate-anticoagulated blood. Avoid clotted samples as they reduce yield.
For best results, process blood within 4–6 hours at room temperature. Samples stored at 4°C can be processed within 24 hours, but viability may decrease.
Yes, Lymphobio can be adjusted to densities up to 1.2 g/mL by adding sterile water or concentrated stock solutions for specialized separations (e.g., subcellular particles).
Yes, Lymphobio can be used with automated PBMC isolation systems such as Sepax or Accuspin tubes. Consult your system's manual for specific protocols.
Normal human serum, AB serum, plasma proteins for cell culture and diagnostics
Fetal bovine serum, FBS low IgG, horse serum for research applications
Automated live-cell imaging for real-time PBMC monitoring and analysis
Plates, flasks, media bottles from innoME GmbH
Contact our sales team for individual offers, bulk discounts, and technical consultation
Why SeamlessBio:
Premium quality reagents • Fast delivery • Technical support • Complete documentation (CoA)
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The Lymphocyte Separation Solution
is specially designed for the efficient isolation of peripheral blood mononuclear cells (PBMCs). This solution provides a reliable method to separate lymphocytes from other blood components, ensuring high purity and viability of the isolated cells. Moreover, it is easy to use and consistently yields high-quality results, making it an essential tool for research labs and clinical applications.
This solution operates on the principle of density gradient centrifugation. When whole blood mixes with the Lymphocyte Separation Solution
, it creates a gradient that allows lymphocytes to separate from plasma and red blood cells. As a result, researchers can retrieve high yields of viable PBMCs efficiently. This process is crucial for various applications, including immunological research and drug development.
The Lymphocyte Separation Solution
plays a pivotal role in numerous research fields. Scientists use isolated PBMCs for studies related to immunology, oncology, and infectious diseases. Because these cells are vital for understanding immune responses, the ability to isolate them with this solution significantly enhances the effectiveness of experimental procedures. Thus, it offers researchers a reliable way to conduct their studies accurately and efficiently.
The Lymphocyte Separation Solution by Lymphobio is specifically designed to isolate peripheral blood mononuclear cells (PBMCs) with high efficiency and purity. With a carefully adjusted density of 1.077 g/mL, it allows for precise separation of mononuclear cells from whole blood. This density is optimal to create a clear interface during centrifugation, ensuring reliable yield of lymphocytes and monocytes for downstream applications. The solution’s consistency enhances reproducibility in research, making it an excellent choice for laboratories focused on immunology and cell biology studies.
Lymphobio’s lymphocyte separation solution serves as an excellent Ficoll alternative, offering comparable performance with added purity and sterility benefits. The product undergoes rigorous sterile filtration, minimizing contamination risks during PBMC isolation workflows. This feature is crucial for sensitive applications like cell culture, immunophenotyping, and molecular diagnostics. By replacing traditional Ficoll, this solution reduces potential endotoxin exposure while maintaining excellent gradient separation properties, supporting high-quality cell isolations that meet stringent experimental standards.
Manufactured under strict quality control measures, the Lymphocyte Separation Solution from Lymphobio delivers consistent physicochemical properties batch after batch. This consistency is critical for reproducible research outcomes and clinical applications. The solution’s chemical composition is balanced to preserve cell viability and functionality during the separation process. Additionally, it is compatible with a wide range of sample volumes and centrifugation protocols, providing flexibility and ease of use for researchers and clinicians alike. Trust Lymphobio for dependable performance in your immunological studies.
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