Human BSEP Vesicle Kit (HEK293-derived) – Bile Salt Export Pump Assay | 100 Reactions
Human BSEP Vesicle Kit (HEK293-derived) – Bile Salt Export Pump Assay | 100 Reactions

Human BSEP Vesicle Kit (HEK293-derived)

The Human BSEP Vesicle Kit provides inside-out membrane vesicles isolated from HEK293 cells transiently overexpressing the Bile...
Vendor: SeamlessBio
SKU: C4PVK007
Availability: In stock
€1.450,00
€1.450,00
Test: 100
Subtotal: €1.450,00
Human BSEP Vesicle Kit (HEK293-derived) – Bile Salt Export Pump Assay | 100 Reactions

Human BSEP Vesicle Kit (HEK293-derived)

€1.450,00
Human BSEP Vesicle Kit (HEK293-derived) – Bile Salt Export Pump Assay | 100 Reactions

Human BSEP Vesicle Kit (HEK293-derived)

€1.450,00
Test: 100
Description
Description
x
The Human BSEP Vesicle Kit provides inside-out membrane vesicles
isolated from HEK293 cells transiently overexpressing the Bile Salt Export
Pump (BSEP / ABCB11). BSEP is the primary hepatic efflux transporter for
bile salts and a critical target in drug-induced cholestasis (DILI) risk
assessment. The kit is compliant with FDA (2017) and EMA (2013) DDI guidance.
 
Manufactured by Cell4Pharma.
Available exclusively in the D-A-CH region via SeamlessBio.

Transporter Profile 

Name: BSEP, Bile Salt Export Pump, ABCB11, ATP-binding cassette sub-family B member 11
Gene: ABCB11
ATP/AMP ratio: >10 (guaranteed)
 
Tissue expression: Canalicular plasma membrane of hepatocytes
Diseases: Inherited and drug-induced cholestasis (DILI)
 
Known substrates:  Taurocholate, pravastatin, taurolitocholyl 3-sulfate, conjugated monovalent bile salts
In stock: Human, cat, and dog BSEP

Kit Contents (100 Reactions)

  • Vesicle vial (100 reactions), BSEP-overexpressing HEK293-derived
  • AMP-ATP solution (100 mM), Cell4Pharma
  • Assay buffer, Cell4Pharma

Applications

  • Bile salt transport inhibition studies (cholestasis risk assessment)
  • Drug-induced liver injury (DILI) screening
  • Drug-drug interaction (DDI) studies per FDA/EMA guidance
  • Substrate identification for hepatic efflux transporters
  • High-throughput screening (radiolabel, fluorescent, LC-MS detection)

Regulatory Compliance

BSEP inhibition is a well-established mechanism of drug-induced
cholestatic liver injury. Assessment of BSEP inhibition is recommended in FDA (2017) and EMA (2013) DDI guidelines as part of the transporter characterization package for new molecular entities (NMEs) entering clinical development.

Technical Specifications

Host cell: HEK293 (human embryonic kidney)
Vesicle type: Inside-out membrane vesicles
Format: 100 reactions per kit
Detection: Radiolabel / fluorescent / LC-MS compatible
Storage: –80 °C

Cross-References & Related Products

  • Human Control Vesicle Kit (C4PVK009) – recommended as negative control
  • Human MRP2 Vesicle Kit (C4PVK002) – for parallel hepatic efflux assessment
  • Human BCRP Vesicle Kit (C4PVK006) – for complete efflux transporter panel
  • ciPTEC-OAT1 Assay-Ready Cells – for cell-based nephrotoxicity studies
  • Human Serum AB Male OTC (SeamlessBio) – for ciPTEC culture medium supplementation
All products available on request.
Contact: info@seamlessbio.de | Phone: +49 851 37932226

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