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Normal Goat Serum (NGS) is the most frequently used blocking serum in immunohistochemistry and immunofluorescence. Since anti-goat secondary antibodies are among the most commonly used secondary antibodies in research, NGS is essential in virtually all IHC, IFA, and ELISA protocols using goat-derived secondary antibodies.
| Parameter | Specification |
|---|---|
| Species | Capra hircus (domestic goat) |
| Designation | Normal Goat Serum (NGS) |
| Sterile filtration | 0.2 µm |
| Origin | EU |
| Storage | -20 °C (long-term) | +4 °C (short-term) |
| Shelf life | Min. 12 months from manufacture |
| Pack sizes | 1 mL, 5 mL, 10 mL, 50 mL, 100 mL |
| Documentation | CoA, CoO, MSDS |
| Application | Description |
|---|---|
| IHC Standard Blocking | 2–10% NGS in PBS as universal blocking reagent before anti-goat secondary antibodies |
| Immunofluorescence (IFA) | Background reduction with fluorescence-conjugated goat secondary antibodies |
| ELISA | Blocking in assays with goat-specific primary or secondary antibodies |
| Multiplex Assays | Goat-specific block in multiplex IHC/IF protocols |
SeamlessBio supplies speciality animal sera — all sterile filtered, with full documentation:
| Species | Typical Application | Availability |
|---|---|---|
| Rat Serum | Immunoassay, blocking, rat primary cells | ✅ In stock |
| Mouse Serum (NMS) | ELISA, Western blot, hybridoma | ✅ In stock |
| Rabbit Serum (NRS) | IHC, IFA, ELISA, blocking | ✅ In stock |
| Sheep Serum (NSS) | IHC, complement, anti-sheep secondary | ✅ In stock |
| Goat Serum (NGS) | IHC standard, IFA, ELISA, multiplex | ✅ In stock |
| Chicken Serum (NCS) | IgY assays, cross-reactivity, veterinary | ✅ In stock |
| Pig Serum | Preclinical, porcine primary cells | ✅ In stock |
| Horse Serum (DHS) | PC-12, hybridoma, insect cells | ✅ In stock |
| Guinea Pig Serum | Complement fixation test (CFT) | ✅ On request |
SDS, CoA and further documents on request: info@seamlessbio.de | +49 851 37932226
Anti-goat secondary antibodies are cost-effective, highly specific, and available in many fluorescence conjugates. NGS blocks endogenous goat IgG binding sites in tissue — prevents non-specific secondary antibody binding.
NGS when goat secondary antibodies are used. Donkey serum (NDS) when donkey secondary antibodies are employed. Mixed protocols available on request.
Yes — info@seamlessbio.de | +49 851 37932226
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