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FBS Ultra Low IgG is fetal bovine serum chromatographically depleted of bovine immunoglobulins to below 5 µg/mL — over 100× lower than standard FBS which contains 200–800 µg/mL bovine IgG. In hybridoma culture and monoclonal antibody production, bovine IgG in standard FBS contaminates culture supernatants, competes with the antibody of interest during protein A/G purification, and elevates background in IgG-detection assays. Ultra Low IgG FBS eliminates this interference without affecting albumin, transferrin, or growth factor content. EU and/or US origin, 0.1 µm sterile filtered.
| Application | Protocol | Note |
|---|---|---|
| Hybridoma culture | Replace standard FBS 1:1 at same concentration (5–20%) | Eliminates bovine IgG contamination in mAb supernatant |
| Monoclonal antibody production | 5–10% in hybridoma production medium throughout culture | Protein A/G purification yield and purity improved |
| IgG ELISA / Western blot development | Ultra Low IgG FBS as negative control matrix or assay diluent | Eliminates bovine IgG cross-reactivity with anti-human/mouse IgG secondaries |
| Immunoprecipitation (IP / Co-IP) | Culture cells in Ultra Low IgG FBS before lysis and IP | Bovine IgG co-precipitation with protein A/G beads eliminated |
| Flow cytometry with anti-IgG antibodies | Culture cells before staining | Non-specific Fc receptor binding via bovine IgG prevented |
| Parameter | Specification |
|---|---|
| IgG content | Ultra Low IgG: <5 µg/mL | Low IgG: <50 µg/mL |
| Processing | Chromatographic purification — IgG fraction selectively removed |
| Filtration | 0.1 µm sterile filtered |
| Endotoxin | Tested per lot — LAL method |
| Mycoplasma | Tested — negative |
| BVDV | Tested — negative |
| Origin | EU and/or US |
| Volumes | 100 mL, 500 mL | Bulk on request |
| Storage | −20 °C | Batch reservation up to 6 weeks |
| Documentation | CoA, CoO, MSDS included |
| Options | Heat inactivated on request | Gamma irradiated on request |
Ultra Low IgG FBS contains less than 5 µg/mL bovine IgG — the highest depletion grade, equivalent to leading international standards. Low IgG FBS contains less than 50 µg/mL. For hybridoma culture and protein A/G purification, Ultra Low IgG is recommended. For general IgG assay development where reduced background is sufficient, Low IgG is often adequate.
No — chromatographic purification removes only the IgG fraction. Albumin, transferrin and growth factors are fully retained. Growth performance is validated on reference cell lines and confirmed equivalent to standard FBS.
Detailed background and application protocols: seamlessbio.de — FBS Ultra Low IgG.
Bulk pricing, batch reservation, GMP documentation: info@seamlessbio.de | +49 851 37932226
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