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DMSO (Dimethyl Sulfoxide, CAS 67-68-5) is the standard cryoprotectant in cell therapy and cell culture. SeamlessBio supplies DMSO at pharmaceutical purity (≥99.9%, sterile filtered) for use as a cryoprotectant in freezing media, as a solubilising agent for poorly soluble compounds, and as a differentiation reagent in cell biology — with full documentation, direct from EU stock in Passau, Germany.
As a direct partner for biological raw materials, SeamlessBio offers DMSO as part of a complete cryopreservation package: DMSO + HSA 20% from a single source, consistent documentation quality, one point of contact.
| Parameter | Specification |
|---|---|
| Purity | ≥99.9% (GC) |
| Form | Sterile solution |
| Sterilisation | Sterile filtered (0.2 µm) |
| Endotoxin | ≤1 EU/mL (LAL method) |
| Mycoplasma | Tested negative |
| Water content | ≤0.1% (Karl Fischer) |
| Quality standard | USP-compliant, Cell Culture Grade |
| Melting point | +18.5 °C (liquid at RT) |
| Storage | +15 to +25 °C, protected from light |
| Packaging | Glass vial (DMSO-resistant) |
| Documentation | CoA, MSDS, sterility test |
DMSO penetrates the cell membrane and displaces intracellular water — preventing ice crystal formation during freezing and protecting the cell membrane from mechanical damage. Combined with an extracellular protective protein (typically HSA 20%), it provides a complete cryopreservation protocol for therapeutic cells.
The standard concentration in freezing media for cell therapy products is 10% DMSO final — combined with 1–5% HSA from a 20% stock solution.
DMSO is the standard cryoprotectant for CAR-T cells, NK cells, MSCs, haematopoietic stem cells (HSC), and iPSCs. Typical freezing protocol: 10% DMSO + 2–5% HSA in serum-free base medium or PBS. SeamlessBio supplies both components — DMSO and HSA 20% — from a single source with matched documentation.
For cryopreservation of established cell lines (CHO, HEK293, Vero, Jurkat, HeLa) in research laboratories. Standard protocol: 10% DMSO in complete medium or 90% FBS / 10% DMSO.
DMSO is used as a polar aprotic solvent for preparing drug stock solutions in pharmacology, toxicology, and compound screening. Typical working concentration: 0.1–1% in cell culture medium.
At defined concentrations (≥1%), DMSO acts as a differentiation reagent for various cell lines — particularly in neural and erythroid differentiation in basic research.
For a complete, documented cryopreservation process in cell therapy, we recommend the combination:
| Component | Function | Product |
|---|---|---|
| DMSO 10% | Intracellular — prevents ice crystal formation | DMSO ≥99.9% (this product) |
| HSA 20% (1–5% final) | Extracellular — membrane protection, osmotic stabilisation | HSA 20% Solution |
| Base medium | Carrier (PBS, PlasmaLyte, serum-free medium) | On request |
Both components — DMSO and HSA — are available from SeamlessBio with consistent documentation quality (CoA, CoO, MSDS, sterility test). Combined orders receive priority processing.
Request the documents by mail: info@seamlessbio.de
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