BSA Low Endotoxin <5 EU/mg | ≥99% Purity | US Origin | Powder

High-Quality Bovine Serum Albumin for Reliable Research

SeamlessBio Bovine Serum Albumin (BSA) Low Endotoxin is a highly purified plasma protein with a purity of at least 96% and strictly controlled endotoxin content below 5 EU/mg. It is sourced from healthy, traceable cattle herds in Australia, New Zealand, or the USA and processed under certified conditions.

Ideal for serum-free cell culture media, ELISA blocking, Western blot, protein quantification, PCR stabilization, and enzyme assays.

What is BSA Low Endotoxin?

Bovine Serum Albumin (BSA) is the most abundant protein in bovine plasma, accounting for approximately 60% of total protein. As a monomeric protein with a molecular weight of 66.5 kDa and 583 amino acids, BSA possesses exceptional properties:

• High water solubility – up to 40% (w/v) soluble
• Stabilizing – protects enzymes, antibodies, and growth factors from denaturation
• Binding capacity – binds fatty acids, hormones, steroids, metal ions
• Non-antigenic – minimal immunoreactivity
• Cost-effective – most affordable high-purity protein source for research

Low Endotoxin BSA is manufactured through additional purification steps such as ion exchange chromatography and diafiltration to reduce endotoxin contamination to below 5 EU/mg. This is essential for:

• Cell cultures – Endotoxin activates TLR4 and triggers proinflammatory cytokines
• Immunoassays – Interference with antibody binding
• Serum-free media – No FBS endotoxins present as buffer

Technical Specifications

BSA Grades Comparison – Which for Which Application?

Recommendation: For cell culture and sensitive immunoassays, Low Endotoxin BSA offers the optimal balance between performance and cost.

Applications

1. Cell Culture Supplementation

BSA is a standard supplement in serum-free media:

• Protein carrier: Transports fatty acids, vitamins (A, D, E), hormones (insulin, thyroid hormones)
• Stabilization: Protects cells from mechanical stress caused by shear forces during pipetting
• Antioxidant: Binds free radicals and reduces oxidative stress
• Osmolarity: Contributes to physiological osmolality (~280-310 mOsm/kg)
• Typical concentration: 0.1 to 5 g/L (0.01 to 0.5% w/v)

Suitable cell lines:

• CHO (Chinese Hamster Ovary) – Bioprocessing, antibody production
• HEK293 (Human Embryonic Kidney) – Virus production, transfection
• Vero (African Green Monkey Kidney) – Vaccine production
• MDCK (Madin-Darby Canine Kidney) – Influenza vaccine
• Sf9/Sf21 (Spodoptera frugiperda) – Baculovirus expression system
• Primary cells – Fibroblasts, hepatocytes, neurons

2. ELISA & Immunoassays

Blocking reagent:

• Blocks nonspecific binding sites on microplates
• Typical concentration: 1 to 5% BSA in PBS or TBS
• Incubation: 1 to 2 hours at room temperature or overnight at 4°C
• Advantage over milk powder: No phosphoproteins, important for anti-phospho antibodies

Sample diluent:

• Diluent for samples and antibodies
• Reduces matrix effects
• Typical: 0.1 to 1% BSA in assay buffer

3. Western Blot

Blocking buffer:

• 5% BSA in TBST (TBS + 0.1% Tween-20)
• Preferred for phosphoproteins, as milk contains phosphorylated casein
• Incubation: 1 hour at room temperature

Antibody dilution:

• 1 to 3% BSA in TBST
• Stabilizes antibodies during long incubations

4. Protein Quantification (Standard)

BSA is THE standard for protein assays:

• Bradford Assay: BSA shows strong binding to Coomassie dye
• BCA Assay: BSA as reference for bicinchoninic acid-based quantification
• Lowry Assay: Classic standard
• A280 (UV): Known extinction coefficient (E^1% = 6.7)

Standard curve: Typically 0 to 2000 µg/ml BSA

5. PCR & Molecular Biology

• Enzyme stabilization: Taq polymerase, restriction enzymes, ligases remain active longer
• Increased PCR yield: Especially with impure templates containing genomic DNA and inhibitors
• Typical concentration: 0.1 to 0.4 mg/ml (100 to 400 µg/ml)
• Nuclease-free: SeamlessBio BSA is tested RNase/DNase-free

6. Enzyme Assays

• Carrier protein: Prevents adsorption of enzymes to vessel walls
• Stabilization during storage: Enzymes in BSA solution (0.1 to 1 mg/ml) retain activity longer
• Examples: Proteases, kinases, phosphatases, oxidoreductases

7. IVD & Diagnostics

• Calibration standards: Known BSA concentrations for analyzer calibration
• Quality control: QC material for clinical chemistry analyzers
• Reagent stabilizer: In commercial diagnostic kits

Why Low Endotoxin Matters

Endotoxins (lipopolysaccharides, LPS) are components of the outer membrane of gram-negative bacteria. Even in minute amounts they activate:

• TLR4 (Toll-Like Receptor 4) → NFκB signaling → proinflammatory cytokines (TNF-α, IL-1β, IL-6)
• Macrophages & monocytes → Immune activation → Assay interference
• Cell culture disruption → Altered gene expression, apoptosis, reduced growth

Critical thresholds:

• Cell culture: <5 EU/mg BSA (at 1 mg/ml BSA = 5 EU/ml medium)
• Serum-free media: <1 EU/ml total recommended
• Immune cells (macrophages, dendritic cells): <0.1 EU/ml ideal

SeamlessBio BSA Low Endotoxin guarantees <5 EU/mg – tested with LAL (Limulus Amebocyte Lysate) assay according to FDA/USP standards.

Origin & Quality Assurance

Australia, New Zealand, USA – The safest regions:

• BSE/TSE-free: All three regions are OIE-certified as BSE-Negligible Risk
• Strict animal husbandry: Grass-fed, no animal meal feeding
• Veterinary supervision: Ante-mortem and post-mortem inspections
• Traceability: Each batch with Certificate of Origin

Each batch is supplied with:

• ✓ Certificate of Analysis (CoA) – Complete specifications
• ✓ Certificate of Origin (COO) – AU/NZ/US origin documented
• ✓ Endotoxin test certificate (LAL) – <5 EU/mg confirmed
• ✓ Sterility proof (when 0.2 µm filtered)
• ✓ BSE/TSE freedom declaration
• ✓ Safety Data Sheet (SDS)

Available Volumes

Other volumes & bulk orders: Larger quantities (≥1 kg) and custom formats are available upon request. Contact us for a customized quote.

Storage & Handling

Powder (Lyophilized):

• Storage: Room temperature (15-25°C), dry, sealed
• Shelf life: 3 years from manufacture
• After opening: Store at 2-8°C, use within 6 months

Solution (30% w/v):

• Storage: 2-8°C in refrigerator
• Shelf life: 2 years from manufacture
• Do not freeze – may lead to protein aggregation

Dissolving powder:

1. Add slowly to water or buffer (PBS, TBS)
2. Swirl gently, do not vortex to avoid foaming
3. Dissolve at room temperature or 37°C to accelerate the process
4. Adjust pH to 7.0 to 7.4 with NaOH/HCl if necessary
5. Sterile filter with 0.2 µm filter for cell culture

Why SeamlessBio for BSA Low Endotoxin?

• BSE/TSE-free origin from AU/NZ/US, OIE-certified
• Guaranteed <5 EU/mg, LAL-tested with each batch
• High purity ≥96%, electrophoresis validated
• Monomers ≥95%, minimal aggregates
• RNase/DNase-free, ideal for molecular biology
• Fast EU delivery, warehouse in Germany, 1-3 days
• Free samples, 10g test volume for qualified customers
• Technical support from our life science team for optimization

Purity and Quality Standards

Our Bovine Serum Albumin powder is manufactured to meet rigorous quality standards, ensuring a purity level of ≥99%. This high purity guarantees that the protein is free from contaminants and impurities that may interfere with sensitive laboratory applications. Each batch is carefully tested for consistency and reliability, making it ideal for biochemical research, diagnostics, and pharmaceutical uses. The superior purity also supports reproducibility in experimental results and enhances overall product performance.

Low Endotoxin Levels for Sensitive Applications

With endotoxin levels below 5 EU/mg, this Bovine Serum Albumin powder is designed for applications requiring minimal endotoxin interference. Low endotoxin levels are critical in cell culture, immunoassays, and therapeutic formulations where endotoxins can affect cell viability or cause unwanted immune responses. Our product’s controlled endotoxin content ensures safer and more accurate outcomes in your experimental protocols and manufacturing processes.

US Origin and Reliable Powder Form

Sourced and manufactured in the United States, this Bovine Serum Albumin powder adheres to strict regulatory and quality control procedures. The powder form provides excellent stability and ease of handling, allowing for convenient storage and precise measurement for laboratory use. The US origin guarantees traceability and adherence to industry best practices, giving researchers confidence in the product’s integrity and consistency throughout their work.