BSA Fatty Acid Free <0.01% | ≥99% Purity | US Origin | Powder

Premium Bovine Serum Albumin Powder for Laboratory and Research Use

SeamlessBio Bovine Serum Albumin Fatty Acid Free is specially purified to remove bound fatty acids that are naturally present in standard BSA. With ≥98% purity and <5 EU/mg endotoxin, it is the first choice for fatty acid-sensitive cell lines (CHO, Vero, MDCK) and lipid metabolism studies.

Why Fatty Acid Free BSA?

Standard BSA contains naturally bound fatty acids (~0.5-2 mol FA per mol BSA), primarily:

• Palmitate (C16:0) – Saturated fatty acid
• Stearate (C18:0) – Saturated fatty acid
• Oleate (C18:1) – Monounsaturated fatty acid
• Linoleate (C18:2) – Polyunsaturated fatty acid

Problems in certain applications:

• Cell culture: CHO, Vero, MDCK are sensitive to exogenous fatty acids → altered growth rates, compromised product quality
• Lipid metabolism studies: Native FA interfere with measurements of lipid transporters, beta-oxidation, lipoprotein metabolism
• Hormone assays: FA influence steroid binding (testosterone, estrogen, cortisol)
• Drug-binding studies: FA compete for albumin binding sites

Solution: Fatty Acid Free BSA eliminates these problems through charcoal delipidation (activated charcoal treatment) → >99% FA removal.

Technical Specifications

Standard vs. Fatty Acid Free BSA

Applications

1. CHO/Vero/MDCK Cell Culture

Why these cell lines need Fatty Acid Free BSA:

CHO (Chinese Hamster Ovary):

• Biomanufacturing of monoclonal antibodies (mAbs) and recombinant proteins
• Exogenous fatty acids disrupt glycosylation and product quality
• FDA/EMA prefer FA-free supplements for GMP production
• Typical concentration: 0.5-2 g/L in serum-free media

Vero (African Green Monkey Kidney):

• Vaccine production (Polio, Rabies, Rotavirus, Dengue)
• WHO-certified cell line for human vaccines
• FA-free conditions are essential for regulatory compliance
• Improved virus titers with FA-free BSA

MDCK (Madin-Darby Canine Kidney):

• Influenza vaccine production (seasonal and pandemic vaccines)
• FDA prefers FA-free supplements
• Higher HA (hemagglutinin) yield

2. Lipid Metabolism & Fatty Acid Uptake Studies

Studies on lipid transporters:

• CD36 (Fatty Acid Translocase): Uptake assays without background from native FA
• FATP (Fatty Acid Transport Protein): Specific FA uptake studies
• FABPpm (Plasma Membrane Fatty Acid Binding Protein): Binding studies

Beta-oxidation assays:

• Measurement of mitochondrial FA oxidation
• No interference from BSA-bound FA
• Use of radiolabeled FA (e.g., ¹⁴C-palmitate)

Lipoprotein metabolism:

• HDL/LDL studies
• Apolipoprotein binding
• CETP (Cholesteryl Ester Transfer Protein) activity

Adipocyte differentiation:

• 3T3-L1 preadipocytes → adipocytes
• Controlled FA addition for lipid accumulation
• Oil Red O staining without background

3. Hormone-Binding Studies

Thyroid hormone (T3, T4) binding:

• FA compete for albumin binding sites
• Fatty Acid Free BSA enables precise binding studies
• Important for thyroid diagnostics

Steroid hormone assays:

• Estrogen, testosterone, cortisol, progesterone
• FA influence protein binding
• Important for endocrinology research

Drug-protein binding studies:

• Pharmacokinetics studies
• Competition between drug and FA for albumin binding sites
• Important for drug development

4. Serum-Free Media Formulation

• Precise control over lipid composition
• Targeted addition of defined fatty acids (e.g., oleic acid, linoleic acid)
• Reproducible cell culture conditions
• GMP-compliant media development

Available Volumes

Other volumes & bulk orders: Larger quantities (≥1 kg) and custom formats are available upon request. Contact us for a customized quote.

Storage & Handling

Storage:

• Room temperature (15-25°C), dry, sealed
• Shelf life: 3 years from manufacture
• After opening: Store at 2-8°C, use within 6 months

Dissolving powder:

1. Add slowly to water or buffer (PBS, TBS)
2. Swirl gently (do not vortex)
3. Dissolve at room temperature or 37°C
4. Adjust pH to 7.0-7.4
5. Sterile filter with 0.2 µm for cell culture

Important: Avoid repeated freeze/thaw cycles as this can lead to protein aggregation.

Origin & Quality Assurance

AU/NZ/US – BSE/TSE-free regions (OIE-certified)

Each batch is supplied with:

• Certificate of Analysis (CoA) – Complete specifications
• Certificate of Origin (COO) – AU/NZ/US origin documented
• Fatty Acid Analysis (GC-MS) – <0.01 mol FA/mol BSA confirmed
• Endotoxin test certificate (LAL) – <5 EU/mg confirmed
• BSE/TSE freedom declaration
• Safety Data Sheet (SDS)

Why SeamlessBio for BSA Fatty Acid Free?

• >99% FA removal – Charcoal delipidation validated
• Purity ≥98% – Higher than standard BSA
• Low Endotoxin <5 EU/mg – Ideal for cell culture
• BSE/TSE-free – AU/NZ/US origin
• Fast EU delivery – Warehouse in Germany, 1-3 days
• Free samples – 10g test volume for qualified customers
• Technical support – For questions about protocols

Overview and Purity of Bovine Serum Albumin

Bovine Serum Albumin (BSA) is a crucial protein widely used in biochemical and molecular biology research. Our BSA powder is fatty acid free at less than 0.01%, ensuring minimal interference in sensitive experiments. With a purity level of ≥99%, this product guarantees consistent and reliable results. Manufactured in the US under strict quality controls, it meets high standards demanded by advanced laboratories worldwide. This product is ideal for applications requiring ultrapure proteins without contamination from fatty acids or other impurities.

Superior Quality and Origin

Originating from the United States, this Bovine Serum Albumin powder is produced following rigorous manufacturing protocols to maintain its integrity and functionality. The fatty acid free specification eliminates variable factors that can affect experimental outcomes, making it suitable for enzyme stabilization, cell culture, and immunoassays. Researchers can trust the consistent quality of this product to enhance assay sensitivity and reproducibility across diverse laboratory protocols.

Applications and Benefits in Scientific Research

Utilizing fatty acid free BSA powder improves the reliability of experiments involving protein binding, blocking, and enzyme stabilization. Its high purity reduces background noise and enhances assay performance in ELISA, Western blotting, and cell culture media. This protein’s stability and solubility make it an invaluable reagent for diagnostic and pharmaceutical research. Choosing this Bovine Serum Albumin ensures that your research benefits from a product designed for precision and optimal lab results.