{"product_id":"mrp4-vesicle-kit","title":"Human MRP4 Vesicle Kit (HEK293 derived) | ABCC4 | Renal \u0026 Hepatic Efflux | 100 Reactions","description":"\u003ch2\u003eHuman MRP4 Vesicle Kit – Inside-Out Membrane Vesicles for Renal Proximal Tubule and Nucleotide Transport Studies\u003c\/h2\u003e\n\n\u003cp\u003eThe Human MRP4 Vesicle Kit (HEK293 derived) provides ready-to-use inside-out membrane vesicles for ATP-dependent transport studies of MRP4 (ABCC4). MRP4 is expressed in renal proximal tubules, hepatocytes, platelets, and the blood-brain barrier. It handles a broad range of substrates including cyclic nucleotides, prostaglandins, urate, and nucleotide analogue antiviral and anticancer drugs — making it particularly important for renal DDI risk assessment and antiviral drug development.\u003c\/p\u003e\n\n\u003ch3\u003eTransporter Profile\u003c\/h3\u003e\n\u003cp\u003eMRP4 (ABCC4) transports cyclic nucleotides (cAMP, cGMP), prostaglandins, urate, glutathione conjugates, and nucleotide analogues. It is expressed in renal proximal tubule apical membrane (contributing to urinary excretion), hepatocyte basolateral membrane, and platelets (where it regulates thromboxane A₂\/cAMP signalling). Key substrates: PMEA (adefovir), methotrexate, cAMP, cGMP, leucovorin. Particularly relevant for antiviral nucleoside analogues (tenofovir, adefovir, cidofovir).\u003c\/p\u003e\n\n\u003ch3\u003eTechnical Specifications\u003c\/h3\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTransporter\u003c\/td\u003e\n\u003ctd\u003eHuman MRP4 (Multidrug Resistance-Associated Protein 4) (ABCC4)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eExpression system\u003c\/td\u003e\n\u003ctd\u003eHEK293 (transient overexpression)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eVesicle type\u003c\/td\u003e\n\u003ctd\u003eInside-out membrane vesicles\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReactions per kit\u003c\/td\u003e\n\u003ctd\u003e100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eATP\/AMP transport ratio\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection compatibility\u003c\/td\u003e\n\u003ctd\u003eRadiolabel, LC-MS\/MS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e−80 °C (supplied on dry ice)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit contents\u003c\/td\u003e\n\u003ctd\u003eVesicles, ATP, AMP, reaction buffer, wash buffer, CoA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eManufacturer\u003c\/td\u003e\n\u003ctd\u003eCell4Pharma (Netherlands)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDistributor DACH\u003c\/td\u003e\n\u003ctd\u003eSeamlessBio GmbH, Passau\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\n\u003ch3\u003eApplications\u003c\/h3\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication\u003c\/th\u003e\n\u003cth\u003eDescription\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAntiviral drug renal excretion studies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNucleotide analogue antivirals (tenofovir, adefovir, cidofovir) are MRP4 substrates. MRP4 inhibition studies assess DDI risk for renal accumulation and nephrotoxicity.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRenal DDI risk assessment\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMRP4 at the renal proximal tubule apical membrane mediates urinary drug excretion. Inhibition can cause renal accumulation of substrates — relevant for dose adjustment and combination therapy.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProstaglandin\/cyclic nucleotide efflux\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eProstaglandins (PGE₁, PGE₂) are endogenous MRP4 substrates. MRP4 inhibition in platelets can alter cAMP levels and affect platelet activation.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\n\u003ch3\u003eAssay Principle\u003c\/h3\u003e\n\u003cp\u003eInside-out membrane vesicles present the cytoplasmic face of the transporter to the assay buffer, allowing ATP-dependent substrate uptake into the vesicle lumen. Incubate vesicles with test compound and probe substrate in the presence of ATP (transport) or AMP (background control). Stop reaction with cold wash buffer, separate vesicles by filtration, and quantify accumulated substrate by LC-MS\/MS, radiolabel, or fluorescence. The ATP\/AMP ratio provides the assay window for reliable IC₅₀ determination.\u003c\/p\u003e\n\n\u003ch3\u003eComplete Cell4Pharma Kit Portfolio at SeamlessBio\u003c\/h3\u003e\n\u003cp\u003eOrder all ABC transporter vesicle kits from a single EU source — fast cold-chain delivery, guaranteed ATP\/AMP ratios, full documentation.\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eKit\u003c\/th\u003e\n\u003cth\u003eTransporter\u003c\/th\u003e\n\u003cth\u003eGene\u003c\/th\u003e\n\u003cth\u003eATP\/AMP Ratio\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman BCRP Vesicle Kit\u003c\/td\u003e\n\u003ctd\u003eBCRP\u003c\/td\u003e\n\u003ctd\u003eABCG2\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman BSEP Vesicle Kit\u003c\/td\u003e\n\u003ctd\u003eBSEP\u003c\/td\u003e\n\u003ctd\u003eABCB11\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman MRP1 Vesicle Kit\u003c\/td\u003e\n\u003ctd\u003eMRP1\u003c\/td\u003e\n\u003ctd\u003eABCC1\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman MRP2 Vesicle Kit\u003c\/td\u003e\n\u003ctd\u003eMRP2\u003c\/td\u003e\n\u003ctd\u003eABCC2\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman MRP3 Vesicle Kit\u003c\/td\u003e\n\u003ctd\u003eMRP3\u003c\/td\u003e\n\u003ctd\u003eABCC3\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman MRP4 Vesicle Kit\u003c\/td\u003e\n\u003ctd\u003eMRP4\u003c\/td\u003e\n\u003ctd\u003eABCC4\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman MRP5 Vesicle Kit\u003c\/td\u003e\n\u003ctd\u003eMRP5\u003c\/td\u003e\n\u003ctd\u003eABCC5\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman MRP8 Vesicle Kit\u003c\/td\u003e\n\u003ctd\u003eMRP8\u003c\/td\u003e\n\u003ctd\u003eABCC11\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman Pgp Vesicle Kit\u003c\/td\u003e\n\u003ctd\u003eP-gp \/ MDR1\u003c\/td\u003e\n\u003ctd\u003eABCB1\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman Pgp + FluoPgp Kit\u003c\/td\u003e\n\u003ctd\u003eP-gp \/ MDR1\u003c\/td\u003e\n\u003ctd\u003eABCB1\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman Control Vesicle Kit\u003c\/td\u003e\n\u003ctd\u003e—\u003c\/td\u003e\n\u003ctd\u003eHEK293 empty vector\u003c\/td\u003e\n\u003ctd\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\n\u003ch3\u003eQuality \u0026amp; Regulatory Compliance\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eProduced by \u003cstrong\u003eCell4Pharma\u003c\/strong\u003e — specialist manufacturer of ABC transporter vesicle systems\u003c\/li\u003e\n\u003cli\u003eGuaranteed ATP\/AMP transport ratio \u003cstrong\u003e\u0026gt;5\u003c\/strong\u003e per lot\u003c\/li\u003e\n\u003cli\u003eLarge, uniform batches — maximum lot-to-lot consistency\u003c\/li\u003e\n\u003cli\u003eLot-specific QC report + data sheet included with every kit\u003c\/li\u003e\n\u003cli\u003eAligned with \u003cstrong\u003eFDA DDI Guidance (2020)\u003c\/strong\u003e and \u003cstrong\u003eEMA DDI Guideline (2013\/2022)\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eWhy Order via SeamlessBio?\u003c\/h3\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eAdvantage\u003c\/th\u003e\n\u003cth\u003eDescription\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e✅ EU warehouse stock\u003c\/td\u003e\n\u003ctd\u003eFast cold-chain delivery across Germany, Austria, Switzerland and the EU\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e✅ Direct contact\u003c\/td\u003e\n\u003ctd\u003eSpeak to a scientist, not a call centre — in German or English\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e✅ Full documentation\u003c\/td\u003e\n\u003ctd\u003eData sheet, QC report and invoice with every shipment\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e✅ Batch reservation\u003c\/td\u003e\n\u003ctd\u003eReserve a specific lot for your entire project duration\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e✅ Complete portfolio\u003c\/td\u003e\n\u003ctd\u003eAll 11 Cell4Pharma kits from one source — BCRP, BSEP, MRP1–8, Pgp, Control\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\n\u003cp\u003eOrder or request pricing: \u003ca href=\"mailto:info@seamlessbio.de\"\u003einfo@seamlessbio.de\u003c\/a\u003e | +49 851 37932226\u003c\/p\u003e\n\n\u003ch3\u003eFrequently Asked Questions\u003c\/h3\u003e\n\u003ch4\u003eIs MRP4 particularly relevant for specific drug classes?\u003c\/h4\u003e\n\u003cp\u003eYes — MRP4 is most important for: (1) nucleotide analogue antivirals (tenofovir, adefovir, cidofovir) where renal MRP4 mediates excretion and DDI risk, (2) NSAIDs and COX inhibitors via prostaglandin efflux, and (3) methotrexate and antifolates.\u003c\/p\u003e","brand":"Cell4Pharma","offers":[{"title":"100","offer_id":58031509406077,"sku":"C4PVK010","price":1450.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0916\/3338\/5853\/files\/MRP4-1-scaled.jpg?v=1776025627","url":"https:\/\/shop.seamlessbio.de\/en\/products\/mrp4-vesicle-kit","provider":"SeamlessBio","version":"1.0","type":"link"}