{"product_id":"mrp2-vesicle-kit","title":"Human MRP2 Vesicle Kit (HEK293 derived) | ABCC2 | Biliary Excretion DDI Assay | 100 Reactions","description":"\u003ch2\u003eHuman MRP2 Vesicle Kit – Inside-Out Membrane Vesicles for Biliary Drug Excretion and DDI Studies\u003c\/h2\u003e\n\n\u003cp\u003eThe Human MRP2 Vesicle Kit (HEK293 derived) provides ready-to-use inside-out membrane vesicles for ATP-dependent transport studies of Multidrug Resistance-Associated Protein 2 (MRP2 \/ ABCC2). With a guaranteed ATP\/AMP ratio of \u0026gt;20 — the highest in the Cell4Pharma vesicle kit range — this kit delivers exceptional assay sensitivity for IC₅₀ determination and substrate identification. MRP2 is co-expressed with BSEP at the hepatocyte canalicular membrane and is a key determinant of biliary drug excretion.\u003c\/p\u003e\n\n\u003ch3\u003eTransporter Profile\u003c\/h3\u003e\n\u003cp\u003eMRP2 (ABCC2) is expressed at the canalicular membrane of hepatocytes, brush-border membrane of renal proximal tubules, and intestinal epithelium. It mediates ATP-dependent export of glucuronide, sulfate, and glutathione conjugates into bile and urine. EMA recommends MRP2 investigation for compounds that undergo significant glucuronidation or sulfation. Key substrates: E217βG, GSSG, methotrexate, irinotecan (SN-38-glucuronide), pravastatin. Known inhibitors: MK-571, cyclosporine A, probenecid.\u003c\/p\u003e\n\n\u003ch3\u003eTechnical Specifications\u003c\/h3\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTransporter\u003c\/td\u003e\n\u003ctd\u003eHuman MRP2 (Multidrug Resistance-Associated Protein 2) (ABCC2)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eExpression system\u003c\/td\u003e\n\u003ctd\u003eHEK293 (transient overexpression)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eVesicle type\u003c\/td\u003e\n\u003ctd\u003eInside-out membrane vesicles\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReactions per kit\u003c\/td\u003e\n\u003ctd\u003e100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eATP\/AMP transport ratio\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection compatibility\u003c\/td\u003e\n\u003ctd\u003eRadiolabel (³H-E217βG), LC-MS\/MS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e−80 °C (supplied on dry ice)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit contents\u003c\/td\u003e\n\u003ctd\u003eVesicles, ATP, AMP, reaction buffer, wash buffer, CoA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eManufacturer\u003c\/td\u003e\n\u003ctd\u003eCell4Pharma (Netherlands)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDistributor DACH\u003c\/td\u003e\n\u003ctd\u003eSeamlessBio GmbH, Passau\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\n\u003ch3\u003eApplications\u003c\/h3\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication\u003c\/th\u003e\n\u003cth\u003eDescription\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBiliary drug excretion profiling\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eQuantify MRP2-mediated hepatic canalicular efflux of glucuronide\/sulfate drug conjugates — essential for prediction of biliary clearance and enterohepatic circulation.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eEMA-recommended DDI assessment\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eEMA 2022 guideline recommends MRP2 characterisation for compounds with significant hepatic glucuronidation or sulfation. IC₅₀ data from vesicle assays is accepted.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRenal tubular efflux studies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMRP2 in renal proximal tubules contributes to urinary excretion of phase II metabolites. Relevant for renal DDI risk assessment.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eEnterohepatic recycling studies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMRP2 in intestinal epithelium contributes to enteric efflux and shapes the enterohepatic circulation of glucuronidated drugs.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\n\u003ch3\u003eAssay Principle\u003c\/h3\u003e\n\u003cp\u003eInside-out membrane vesicles present the cytoplasmic face of the transporter to the assay buffer, allowing ATP-dependent substrate uptake into the vesicle lumen. Incubate vesicles with test compound and probe substrate in the presence of ATP (transport) or AMP (background control). Stop reaction with cold wash buffer, separate vesicles by filtration, and quantify accumulated substrate by LC-MS\/MS, radiolabel, or fluorescence. The ATP\/AMP ratio provides the assay window for reliable IC₅₀ determination.\u003c\/p\u003e\n\n\u003ch3\u003eComplete Cell4Pharma Kit Portfolio at SeamlessBio\u003c\/h3\u003e\n\u003cp\u003eOrder all ABC transporter vesicle kits from a single EU source — fast cold-chain delivery, guaranteed ATP\/AMP ratios, full documentation.\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eKit\u003c\/th\u003e\n\u003cth\u003eTransporter\u003c\/th\u003e\n\u003cth\u003eGene\u003c\/th\u003e\n\u003cth\u003eATP\/AMP Ratio\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman BCRP Vesicle Kit\u003c\/td\u003e\n\u003ctd\u003eBCRP\u003c\/td\u003e\n\u003ctd\u003eABCG2\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman BSEP Vesicle Kit\u003c\/td\u003e\n\u003ctd\u003eBSEP\u003c\/td\u003e\n\u003ctd\u003eABCB11\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman MRP1 Vesicle Kit\u003c\/td\u003e\n\u003ctd\u003eMRP1\u003c\/td\u003e\n\u003ctd\u003eABCC1\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman MRP2 Vesicle Kit\u003c\/td\u003e\n\u003ctd\u003eMRP2\u003c\/td\u003e\n\u003ctd\u003eABCC2\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman MRP3 Vesicle Kit\u003c\/td\u003e\n\u003ctd\u003eMRP3\u003c\/td\u003e\n\u003ctd\u003eABCC3\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman MRP4 Vesicle Kit\u003c\/td\u003e\n\u003ctd\u003eMRP4\u003c\/td\u003e\n\u003ctd\u003eABCC4\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman MRP5 Vesicle Kit\u003c\/td\u003e\n\u003ctd\u003eMRP5\u003c\/td\u003e\n\u003ctd\u003eABCC5\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman MRP8 Vesicle Kit\u003c\/td\u003e\n\u003ctd\u003eMRP8\u003c\/td\u003e\n\u003ctd\u003eABCC11\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman Pgp Vesicle Kit\u003c\/td\u003e\n\u003ctd\u003eP-gp \/ MDR1\u003c\/td\u003e\n\u003ctd\u003eABCB1\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman Pgp + FluoPgp Kit\u003c\/td\u003e\n\u003ctd\u003eP-gp \/ MDR1\u003c\/td\u003e\n\u003ctd\u003eABCB1\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman Control Vesicle Kit\u003c\/td\u003e\n\u003ctd\u003e—\u003c\/td\u003e\n\u003ctd\u003eHEK293 empty vector\u003c\/td\u003e\n\u003ctd\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\n\u003ch3\u003eQuality \u0026amp; Regulatory Compliance\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eProduced by \u003cstrong\u003eCell4Pharma\u003c\/strong\u003e — specialist manufacturer of ABC transporter vesicle systems\u003c\/li\u003e\n\u003cli\u003eGuaranteed ATP\/AMP transport ratio \u003cstrong\u003e\u0026gt;20\u003c\/strong\u003e per lot\u003c\/li\u003e\n\u003cli\u003eLarge, uniform batches — maximum lot-to-lot consistency\u003c\/li\u003e\n\u003cli\u003eLot-specific QC report + data sheet included with every kit\u003c\/li\u003e\n\u003cli\u003eAligned with \u003cstrong\u003eFDA DDI Guidance (2020)\u003c\/strong\u003e and \u003cstrong\u003eEMA DDI Guideline (2013\/2022)\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eWhy Order via SeamlessBio?\u003c\/h3\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eAdvantage\u003c\/th\u003e\n\u003cth\u003eDescription\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e✅ EU warehouse stock\u003c\/td\u003e\n\u003ctd\u003eFast cold-chain delivery across Germany, Austria, Switzerland and the EU\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e✅ Direct contact\u003c\/td\u003e\n\u003ctd\u003eSpeak to a scientist, not a call centre — in German or English\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e✅ Full documentation\u003c\/td\u003e\n\u003ctd\u003eData sheet, QC report and invoice with every shipment\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e✅ Batch reservation\u003c\/td\u003e\n\u003ctd\u003eReserve a specific lot for your entire project duration\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e✅ Complete portfolio\u003c\/td\u003e\n\u003ctd\u003eAll 11 Cell4Pharma kits from one source — BCRP, BSEP, MRP1–8, Pgp, Control\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\n\u003cp\u003eOrder or request pricing: \u003ca href=\"mailto:info@seamlessbio.de\"\u003einfo@seamlessbio.de\u003c\/a\u003e | +49 851 37932226\u003c\/p\u003e\n\n\u003ch3\u003eFrequently Asked Questions\u003c\/h3\u003e\n\u003ch4\u003eWhy does MRP2 have the highest ATP\/AMP ratio (\u0026gt;20)?\u003c\/h4\u003e\n\u003cp\u003eThe ATP\/AMP ratio reflects the active transport efficiency of the overexpressed transporter relative to passive diffusion. MRP2's very high ratio (\u0026gt;20) means an exceptional assay window — lower compound concentrations required to see inhibition, more reliable IC₅₀ curves, and higher confidence data for regulatory submissions.\u003c\/p\u003e\n\u003ch4\u003eIs MRP2 required in the FDA DDI submission package?\u003c\/h4\u003e\n\u003cp\u003eFDA does not list MRP2 as a mandatory Tier 1 transporter, but EMA recommends it for compounds with significant glucuronidation. For full regulatory coverage across both agencies, MRP2 data is valuable — particularly for compounds undergoing significant hepatic\/intestinal glucuronidation.\u003c\/p\u003e","brand":"Cell4Pharma","offers":[{"title":"100","offer_id":58031509012861,"sku":"C4PVK010","price":1450.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0916\/3338\/5853\/files\/MRP2-1-scaled.jpg?v=1776025624","url":"https:\/\/shop.seamlessbio.de\/en\/products\/mrp2-vesicle-kit","provider":"SeamlessBio","version":"1.0","type":"link"}