{"product_id":"human-serum-type-ab-male-donor-off-the-clot","title":"Human Serum Type AB Male, Off-the-Clot | Xeno-Free | EU Origin","description":"\u003ch2\u003eHuman Serum Type AB – Male Donors, Off-the-Clot | Xeno-Free | EU\/US Origin\u003c\/h2\u003e\n\u003cp\u003eSeamlessBio Human Serum Type AB (male donors, off-the-clot) is collected exclusively from whole blood of healthy male AB donors at certified collection centres in the EU and USA. Through natural coagulation without anticoagulants (off-the-clot), this serum retains the full native spectrum of growth factors – PDGF, EGF, TGF-β, IGF-1 – released during platelet degranulation.\u003c\/p\u003e\n\u003cp\u003eAs a xeno-free, human-specific alternative to FBS, this serum is the preferred standard in GMP-adjacent workflows for CAR-T, NK cell expansion, ATMP manufacturing, vaccine development, tissue engineering, and in vitro diagnostics (IVD).\u003c\/p\u003e\n\u003ch3\u003eKey Product Benefits\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eType AB – no anti-A or anti-B antibodies, minimal immunoreactivity\u003c\/li\u003e\n\u003cli\u003eMale donors only – eliminates hormonal variability (oestradiol, progesterone)\u003c\/li\u003e\n\u003cli\u003eOff-the-clot – maximum native growth factors (PDGF, EGF, TGF-β, IGF-1)\u003c\/li\u003e\n\u003cli\u003eXeno-free – no bovine antigens, compliant with EMA\/410\/01\u003c\/li\u003e\n\u003cli\u003eEU\/US origin – FDA-registered and EMA-compliant collection centres\u003c\/li\u003e\n\u003cli\u003ePooled lot (200–250 donors) – high lot-to-lot consistency\u003c\/li\u003e\n\u003cli\u003eSterile filtered (0.1–0.2 µm), mycoplasma-negative\u003c\/li\u003e\n\u003cli\u003eVirus testing: HIV-1\/2, HBsAg, anti-HCV, syphilis, NAT\u003c\/li\u003e\n\u003cli\u003eHeat inactivation (56 °C, 30 min) and gamma irradiation available on request\u003c\/li\u003e\n\u003cli\u003eGMP documentation available on request\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eApplications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eCAR-T cell therapy – expansion and activation\u003c\/li\u003e\n\u003cli\u003eNK cell expansion (feeder-free, haematopoietic systems)\u003c\/li\u003e\n\u003cli\u003eMesenchymal stem cells (MSC) from adipose tissue and bone marrow\u003c\/li\u003e\n\u003cli\u003eATMP manufacturing (ancillary material, Ph. Eur. 5.2.12)\u003c\/li\u003e\n\u003cli\u003eVaccine development – human-specific immune response models\u003c\/li\u003e\n\u003cli\u003eTissue engineering – skin, cartilage, cornea\u003c\/li\u003e\n\u003cli\u003eIn vitro diagnostics (IVD) – ELISA matrix, assay development\u003c\/li\u003e\n\u003cli\u003eIslet transplantation research (pancreatic islets)\u003c\/li\u003e\n\u003cli\u003eHLA typing and transplantation research\u003c\/li\u003e\n\u003cli\u003eOncology – tumour spheroid formation, invasion studies\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eTechnical Specifications\u003c\/h3\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct type\u003c\/td\u003e\n\u003ctd\u003eHuman serum, off-the-clot\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBlood group\u003c\/td\u003e\n\u003ctd\u003eAB (no anti-A, no anti-B antibodies)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDonors\u003c\/td\u003e\n\u003ctd\u003eMale only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOrigin\u003c\/td\u003e\n\u003ctd\u003eEU and\/or USA (per lot)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCollection centres\u003c\/td\u003e\n\u003ctd\u003eFDA-registered \/ EMA-compliant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePool size\u003c\/td\u003e\n\u003ctd\u003e200–250 donors per lot\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSterility\u003c\/td\u003e\n\u003ctd\u003e0.1–0.2 µm sterile filtered\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMycoplasma\u003c\/td\u003e\n\u003ctd\u003eNegative (tested)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eVirus testing\u003c\/td\u003e\n\u003ctd\u003eHIV-1\/2 Ag\/Ab, HBsAg, anti-HCV, syphilis, NAT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHeat inactivation\u003c\/td\u003e\n\u003ctd\u003eAvailable on request (56 °C, 30 min)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eGamma irradiation\u003c\/td\u003e\n\u003ctd\u003eAvailable on request (25–45 kGy)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e–20 °C (long-term) \/ +2 to +8 °C (after thawing)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAvailable formats\u003c\/td\u003e\n\u003ctd\u003e100 ml, 500 ml, bulk on request\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIntended use\u003c\/td\u003e\n\u003ctd\u003eResearch Use Only (RUO) | GMP documentation on request\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eDocumentation (per lot)\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eCertificate of Analysis (CoA) – lot-specific\u003c\/li\u003e\n\u003cli\u003eVirus testing protocols (HIV, HBV, HCV, syphilis, NAT)\u003c\/li\u003e\n\u003cli\u003eSterility and mycoplasma test protocol\u003c\/li\u003e\n\u003cli\u003eOrigin and collection centre certificate\u003c\/li\u003e\n\u003cli\u003eSafety Data Sheet (SDS)\u003c\/li\u003e\n\u003cli\u003eGMP documentation package (CoA, virus validation reports, donor certificates) on request\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWho is this product for?\u003c\/h3\u003e\n\u003ch4\u003eATMP Manufacturers \u0026amp; CDMOs\u003c\/h4\u003e\n\u003cp\u003eCAR-T and NK cell products are classified as Advanced Therapy Medicinal Products (ATMPs) under EU Regulation 1394\/2007. Human Serum AB (male, off-the-clot) is the most widely used ancillary material for ex vivo expansion of T and NK cells. SeamlessBio provides lot-specific documentation compliant with Ph. Eur. 5.2.12 and Eudralex Vol. 4, Part IV.\u003c\/p\u003e\n\u003ch4\u003eCROs (Contract Research Organisations)\u003c\/h4\u003e\n\u003cp\u003eFor CROs using human cell models in preclinical testing – e.g. cytotoxicity studies (ISO 10993-5), immunological assays, spheroid formation – Human Serum AB Male Off-the-Clot provides the most physiologically relevant serum matrix. No bovine cross-reactivities, consistent batch quality for reproducible data.\u003c\/p\u003e\n\u003ch4\u003eVaccine Manufacturers \u0026amp; Vaccine Development\u003c\/h4\u003e\n\u003cp\u003eFor the development and testing of vaccines against human pathogens (e.g. influenza, RSV, malaria, SARS-CoV-2), a serum-free or xeno-free medium is preferred. Human Serum AB delivers human-specific growth factors and an antibody-free matrix (blood group AB), ideal for neutralisation assays and immune cell culture.\u003c\/p\u003e\n\u003ch4\u003eTissue Engineering \u0026amp; Cell Therapy Producers\u003c\/h4\u003e\n\u003cp\u003eFor the production of skin grafts, cartilage tissue, or corneal transplants, xeno-free human-specific serum is a prerequisite for clinical translation. Human Serum AB Male Off-the-Clot is the physiologically correct supplement for primary keratinocytes, fibroblasts, chondrocytes, and limbal stem cells.\u003c\/p\u003e\n\u003ch4\u003eDiagnostics Customers (IVD)\u003c\/h4\u003e\n\u003cp\u003eFor the development of immunoassays, ELISA kits, and diagnostics, Human Serum AB is indispensable as a sample matrix. The AB blood group eliminates anti-A\/anti-B interference. Lot-specific quality documentation supports validation per ISO 13485 and the In Vitro Diagnostic Regulation (IVDR 2017\/746).\u003c\/p\u003e\n\u003ch4\u003eMalaria \u0026amp; Infectious Disease Research\u003c\/h4\u003e\n\u003cp\u003eFor cultivation of Plasmodium falciparum and other intraerythrocytic parasites, Human Serum AB (off-the-clot) is the standard supplement. AB specificity prevents blood-group-specific lysis reactions. SeamlessBio delivers batches with complete virus testing protocols for safe BSL-2 handling.\u003c\/p\u003e\n\u003ch3\u003eWhy off-the-clot – and not plasma-derived?\u003c\/h3\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eProperty\u003c\/th\u003e\n\u003cth\u003eOff-the-Clot (OTC)\u003c\/th\u003e\n\u003cth\u003ePlasma-Derived\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eGrowth factors\u003c\/td\u003e\n\u003ctd\u003eHigh (native platelet degranulation)\u003c\/td\u003e\n\u003ctd\u003eModerate (CaCl₂ conversion)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAvailability\u003c\/td\u003e\n\u003ctd\u003eLimited (more donors\/lot)\u003c\/td\u003e\n\u003ctd\u003eMore widely available\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended for\u003c\/td\u003e\n\u003ctd\u003eCAR-T, NK cells, MSC, tissue engineering\u003c\/td\u003e\n\u003ctd\u003eAssay development, diagnostics\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eComplement activity\u003c\/td\u003e\n\u003ctd\u003ePresent (unless heat inactivated)\u003c\/td\u003e\n\u003ctd\u003ePresent (unless heat inactivated)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDonors per lot\u003c\/td\u003e\n\u003ctd\u003e200–250\u003c\/td\u003e\n\u003ctd\u003eFewer donors possible\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eWhy male donors – what is the concrete difference?\u003c\/h3\u003e\n\u003cp\u003eFemale donors have cyclically fluctuating levels of oestradiol and progesterone. These hormones influence cell proliferation, differentiation, and gene expression. In reproducibility-critical experiments – especially in cell therapy – this variability can skew results. Serum from exclusively male donors eliminates this confounding factor. Additionally, female donors who have been pregnant may carry anti-MHC antibodies (TRALI risk) – excluded with male donors.\u003c\/p\u003e\n\u003ch3\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eIs this product suitable for GMP processes?\u003c\/strong\u003e\u003cbr\u003eThe standard product is RUO (Research Use Only). For GMP applications (Phase I\/II, ATMP manufacturing), we offer a GMP documentation package: lot-specific CoA, virus validation reports, collection centre certificates, compliance declaration Ph. Eur. 5.2.12. Please contact us at info@seamlessbio.de.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eHow should the serum be stored after thawing?\u003c\/strong\u003e\u003cbr\u003eStore at +2 to +8 °C after thawing; do not refreeze. Thawed serum is stable for 30 days. Aliquoting before first use is recommended. Turbidity after thawing is normal (lipid precipitation) and does not affect quality.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eWhat concentration is recommended?\u003c\/strong\u003e\u003cbr\u003eStandard: 5–20% in cell culture medium. CAR-T\/NK protocols typically use 2–10%. When switching from FBS: stepwise adaptation recommended (week 1: 5% FBS + 5% human serum; week 2: 100% human serum).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDo you also supply single-donor lots?\u003c\/strong\u003e\u003cbr\u003eYes, on request for metabolic studies or HLA typing experiments.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIs heat inactivation available?\u003c\/strong\u003e\u003cbr\u003eYes. Heat inactivation (56 °C, 30 min) for complement-sensitive applications (haematopoietic cells, immunoassays) and gamma irradiation (25–45 kGy) for maximum viral safety in ATMP processes.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eWhich pack sizes are available?\u003c\/strong\u003e\u003cbr\u003eStandard: 100 ml and 500 ml. Bulk quantities and custom filling on request from 1 litre.\u003c\/p\u003e\n\u003chr\u003e\n\u003cp\u003e\u003cstrong\u003eRequest a sample, GMP documentation, or get a quote:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch1\u003e\n\u003cspan style=\"font-family: -apple-system, BlinkMacSystemFont, 'San Francisco', 'Segoe UI', Roboto, 'Helvetica Neue', sans-serif; font-size: 0.875rem;\"\u003eAsk us for a quote: \u003c\/span\u003e\u003ca title=\"Mail\" href=\"mailto:info@seamlessbio.de\" style=\"font-family: -apple-system, BlinkMacSystemFont, 'San Francisco', 'Segoe UI', Roboto, 'Helvetica Neue', sans-serif; font-size: 0.875rem;\"\u003einfo@seamlessbio.de\u003c\/a\u003e\u003cspan style=\"font-family: -apple-system, BlinkMacSystemFont, 'San Francisco', 'Segoe UI', Roboto, 'Helvetica Neue', sans-serif; font-size: 0.875rem;\"\u003e or directly by Phone: +49 (0) 851 37932226\u003c\/span\u003e\n\u003c\/h1\u003e\n\u003cp\u003e\u003cspan style=\"font-family: -apple-system, BlinkMacSystemFont, 'San Francisco', 'Segoe UI', Roboto, 'Helvetica Neue', sans-serif; font-size: 0.875rem;\"\u003eSee more products:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p1\"\u003e\u003cspan class=\"s1\"\u003e\u003ca href=\"https:\/\/shop.seamlessbio.de\/products\/human-serum-type-ab-mix-eu-us-origin-heat-inactivated\"\u003eHuman Serum AB Mixed Gender\u003c\/a\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p1\"\u003e\u003cspan class=\"s1\"\u003e\u003ca href=\"https:\/\/shop.seamlessbio.de\/products\/humanserum-zellkultur-hitzeinaktiviert\"\u003eHuman Serum Hitzeinaktiviert\u003c\/a\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p1\"\u003e\u003cspan class=\"s1\"\u003e\u003ca href=\"https:\/\/shop.seamlessbio.de\/products\/human-serum-albumin-hsa-us-origin\"\u003eHuman Serum Albumin (HSA)\u003c\/a\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"SeamlessBio","offers":[{"title":"50mL","offer_id":56811953226109,"sku":null,"price":195.0,"currency_code":"EUR","in_stock":false},{"title":"100mL","offer_id":56811953258877,"sku":null,"price":350.0,"currency_code":"EUR","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0916\/3338\/5853\/files\/14_ca52f8d4-ecac-463d-b4b1-6dcfda6b0dfe.png?v=1771692758","url":"https:\/\/shop.seamlessbio.de\/en\/products\/human-serum-type-ab-male-donor-off-the-clot","provider":"SeamlessBio","version":"1.0","type":"link"}