{"product_id":"control-vesicle-kit","title":"Human Control Vesicle Kit (HEK293 derived) | Empty Vector | Negative Control for All ABC Transporter Assays","description":"\u003ch2 id=\"tinymce_scroll_sign\"\u003eComprehensive Overview of the Human Control Vesicle Kit\u003c\/h2\u003e\n\u003cp\u003eThe Human Control Vesicle Kit (HEK293 derived) provides inside-out membrane vesicles prepared from HEK293 cells transfected with empty vector (no transporter). These matched control vesicles are produced using the identical process as all Cell4Pharma transporter vesicle kits — ensuring that any non-specific compound uptake, lipid interactions, or background transport is fully captured and subtracted from assay data. Use of matched control vesicles alongside transporter vesicles is essential for valid substrate classification, reliable IC₅₀ determination, and regulatory-grade data quality.\u003c\/p\u003e\n\u003ch3\u003eTransporter Profile\u003c\/h3\u003e\n\u003cp\u003eControl vesicles contain no overexpressed transporter — they are prepared from the identical HEK293 cell background used for all Cell4Pharma transporter kits, transfected with empty vector only. This matched background ensures that any compound accumulation detected in the ATP condition of control vesicles reflects non-specific membrane partitioning, lipid binding, or endogenous transporter activity — not specific ABC transporter-mediated uptake. The ATP\/AMP-dependent transport ratio for the active transporter kit is calculated by comparing with these matched control vesicles. FDA and EMA guidance specify use of matched control vesicles in vesicular transport assays.\u003c\/p\u003e\n\u003ch3\u003eTechnical Specifications\u003c\/h3\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTransporter\u003c\/td\u003e\n\u003ctd\u003eControl Vesicles (HEK293 empty vector) (HEK293 empty vector — no transporter)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eExpression system\u003c\/td\u003e\n\u003ctd\u003eHEK293 (transient overexpression)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eVesicle type\u003c\/td\u003e\n\u003ctd\u003eInside-out membrane vesicles\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReactions per kit\u003c\/td\u003e\n\u003ctd\u003e100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eATP\/AMP transport ratio\u003c\/td\u003e\n\u003ctd\u003eN\/A — matched control (empty vector)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection compatibility\u003c\/td\u003e\n\u003ctd\u003eMatched to all detection methods (radiolabel, LC-MS\/MS, fluorescence)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e−80 °C (supplied on dry ice)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit contents\u003c\/td\u003e\n\u003ctd\u003eVesicles, ATP, AMP, reaction buffer, wash buffer, CoA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eManufacturer\u003c\/td\u003e\n\u003ctd\u003eCell4Pharma (Netherlands)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDistributor DACH\u003c\/td\u003e\n\u003ctd\u003eSeamlessBio GmbH, Passau\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eApplications\u003c\/h3\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication\u003c\/th\u003e\n\u003cth\u003eDescription\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatched negative control for all ABC transporter assays\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRequired alongside every transporter vesicle kit — BCRP, BSEP, MRP1–8, Pgp. Enables accurate calculation of specific transporter-mediated uptake by subtracting background vesicle accumulation.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eNon-specific binding and lipid partitioning assessment\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHighly lipophilic compounds can partition into membrane lipids independently of transporters. Control vesicles quantify this background for accurate net transport calculation.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRegulatory-grade data generation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFDA and EMA require use of matched control membranes in vesicular transport assays to demonstrate transporter specificity. Control vesicle data must be included in regulatory submission packages.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eAssay Principle\u003c\/h3\u003e\n\u003cp\u003eInside-out membrane vesicles present the cytoplasmic face of the transporter to the assay buffer, allowing ATP-dependent substrate uptake into the vesicle lumen. Incubate vesicles with test compound and probe substrate in the presence of ATP (transport) or AMP (background control). Stop reaction with cold wash buffer, separate vesicles by filtration, and quantify accumulated substrate by LC-MS\/MS, radiolabel, or fluorescence. The ATP\/AMP ratio provides the assay window for reliable IC₅₀ determination.\u003c\/p\u003e\n\u003ch3\u003eComplete Cell4Pharma Kit Portfolio at SeamlessBio\u003c\/h3\u003e\n\u003cp\u003eOrder all ABC transporter vesicle kits from a single EU source — fast cold-chain delivery, guaranteed ATP\/AMP ratios, full documentation.\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eKit\u003c\/th\u003e\n\u003cth\u003eTransporter\u003c\/th\u003e\n\u003cth\u003eGene\u003c\/th\u003e\n\u003cth\u003eATP\/AMP Ratio\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman BCRP Vesicle Kit\u003c\/td\u003e\n\u003ctd\u003eBCRP\u003c\/td\u003e\n\u003ctd\u003eABCG2\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman BSEP Vesicle Kit\u003c\/td\u003e\n\u003ctd\u003eBSEP\u003c\/td\u003e\n\u003ctd\u003eABCB11\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman MRP1 Vesicle Kit\u003c\/td\u003e\n\u003ctd\u003eMRP1\u003c\/td\u003e\n\u003ctd\u003eABCC1\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman MRP2 Vesicle Kit\u003c\/td\u003e\n\u003ctd\u003eMRP2\u003c\/td\u003e\n\u003ctd\u003eABCC2\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman MRP3 Vesicle Kit\u003c\/td\u003e\n\u003ctd\u003eMRP3\u003c\/td\u003e\n\u003ctd\u003eABCC3\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman MRP4 Vesicle Kit\u003c\/td\u003e\n\u003ctd\u003eMRP4\u003c\/td\u003e\n\u003ctd\u003eABCC4\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman MRP5 Vesicle Kit\u003c\/td\u003e\n\u003ctd\u003eMRP5\u003c\/td\u003e\n\u003ctd\u003eABCC5\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman MRP8 Vesicle Kit\u003c\/td\u003e\n\u003ctd\u003eMRP8\u003c\/td\u003e\n\u003ctd\u003eABCC11\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman Pgp Vesicle Kit\u003c\/td\u003e\n\u003ctd\u003eP-gp \/ MDR1\u003c\/td\u003e\n\u003ctd\u003eABCB1\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman Pgp + FluoPgp Kit\u003c\/td\u003e\n\u003ctd\u003eP-gp \/ MDR1\u003c\/td\u003e\n\u003ctd\u003eABCB1\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman Control Vesicle Kit\u003c\/td\u003e\n\u003ctd\u003e—\u003c\/td\u003e\n\u003ctd\u003eHEK293 empty vector\u003c\/td\u003e\n\u003ctd\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eQuality \u0026amp; Regulatory Compliance\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eProduced by \u003cstrong\u003eCell4Pharma\u003c\/strong\u003e — specialist manufacturer of ABC transporter vesicle systems\u003c\/li\u003e\n\u003cli\u003eGuaranteed ATP\/AMP transport ratio \u003cstrong\u003eN\/A (matched control)\u003c\/strong\u003e per lot\u003c\/li\u003e\n\u003cli\u003eLarge, uniform batches — maximum lot-to-lot consistency\u003c\/li\u003e\n\u003cli\u003eLot-specific QC report + data sheet included with every kit\u003c\/li\u003e\n\u003cli\u003eAligned with \u003cstrong\u003eFDA DDI Guidance (2020)\u003c\/strong\u003e and \u003cstrong\u003eEMA DDI Guideline (2013\/2022)\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Order via SeamlessBio?\u003c\/h3\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eAdvantage\u003c\/th\u003e\n\u003cth\u003eDescription\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e✅ EU warehouse stock\u003c\/td\u003e\n\u003ctd\u003eFast cold-chain delivery across Germany, Austria, Switzerland and the EU\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e✅ Direct contact\u003c\/td\u003e\n\u003ctd\u003eSpeak to a scientist, not a call centre — in German or English\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e✅ Full documentation\u003c\/td\u003e\n\u003ctd\u003eData sheet, QC report and invoice with every shipment\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e✅ Batch reservation\u003c\/td\u003e\n\u003ctd\u003eReserve a specific lot for your entire project duration\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e✅ Complete portfolio\u003c\/td\u003e\n\u003ctd\u003eAll 11 Cell4Pharma kits from one source — BCRP, BSEP, MRP1–8, Pgp, Control\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eOrder or request pricing: \u003ca href=\"mailto:info@seamlessbio.de\"\u003einfo@seamlessbio.de\u003c\/a\u003e | +49 851 37932226\u003c\/p\u003e\n\u003ch3\u003eFrequently Asked Questions\u003c\/h3\u003e\n\u003ch4\u003eMust I use control vesicles with every transporter kit?\u003c\/h4\u003e\n\u003cp\u003eYes — matched control vesicles are required for valid assay interpretation. FDA and EMA both specify the use of matched empty-vector or mock-transfected control membranes. Data generated without matched controls is not considered regulatory-grade and may not be accepted in DDI submissions. We recommend ordering one control kit per transporter kit used.\u003c\/p\u003e\n\u003ch4\u003eAre the control vesicles produced from the same cell batch as the transporter vesicles?\u003c\/h4\u003e\n\u003cp\u003eCell4Pharma control vesicles are produced from the identical HEK293 cell line background under the same manufacturing conditions as all transporter vesicle kits — ensuring maximum matching for membrane composition, endogenous transporter expression, and lipid profile.\u003c\/p\u003e\n\u003ch3\u003eWhat is the Human Control Vesicle Kit?\u003c\/h3\u003e\n\u003cp\u003eThe Human Control Vesicle Kit is an essential tool derived from HEK293 cells, designed specifically as an empty vector and negative control for various ABC transporter assays. This kit provides researchers with highly purified control vesicles, ensuring that experiments measuring transporter activity remain accurate and reliable. By utilizing human cell-derived vesicles without any expressed transporter proteins, it helps eliminate background noise and validates assay specificity, making it indispensable for drug discovery and transporter function studies.\u003c\/p\u003e\n\u003ch3\u003eKey Features and Benefits of the Kit\u003c\/h3\u003e\n\u003cp\u003eThe kit includes vesicles prepared from HEK293 cells transfected with an empty vector, serving as an ideal negative control. This assures that any measured transport activity in assays can be confidently attributed to the transporter of interest. The vesicles exhibit high stability and are compatible with a range of substrates and inhibitors, enabling precise characterization of ABC transporters. Additionally, the quality-controlled preparation simplifies experimental workflows, saving time while enhancing reproducibility in your research.\u003c\/p\u003e\n\u003ch3\u003eApplications in ABC Transporter Research\u003c\/h3\u003e\n\u003cp\u003eUsing the Human Control Vesicle Kit is crucial when evaluating efflux transporter activity, including P-glycoprotein, MRP family, and BCRP assays. This negative control provides a baseline to differentiate between specific transporter-mediated transport and nonspecific substrate interactions. It supports assay development, validation, and screening of novel drugs targeting ABC transporters. Overall, the kit improves confidence in data interpretation, contributing to the successful identification of transporter inhibitors or substrates in pharmaceutical development.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Cell4Pharma","offers":[{"title":"100","offer_id":58031523758461,"sku":"C4PVK010","price":1450.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0916\/3338\/5853\/files\/HumanControl-scaled.jpg?v=1776025634","url":"https:\/\/shop.seamlessbio.de\/en\/products\/control-vesicle-kit","provider":"SeamlessBio","version":"1.0","type":"link"}